Epicocconone

• (6S, 9aS ) -3 - ( ( 1Z, 4E, 6E, 8E )-1- hydroxy-3- oxodeca -1 ,4,6,8 - tetraenyl ) - 6 - (hydroxymethyl) -9a- methyl-5, 6- dihydro- 9Ah for - [3,2 -g] isochromene -2 ,9 -dione
• Lightning Fast
• Deep Purple
• Lava Purple
• LavaCell

Template: Infobox chemical / molecular formula search available

Epicocconon is a fluorescent dye from the fungus Epicoccum nigrum.

Properties and Uses

The water-soluble Epicocconon fluoresces slightly greenish ( λem = 520 nm) in its protonated form, after binding of a protein to the maximum fluorescence shifts to a wavelength of 605 nm, the absorption maximum at 395 nm upon addition of a base, a proton is released. The resulting anion loses the ability to fluoresce, however:

The dye can react with primary amines. This is used in biochemistry in the context of a protein characterization to stain proteins, particularly in the quantitation in solutions, for tracking a proteolysis in SDS -PAGE, wherein 2D gel electrophoresis using in- gel digestion and mass spectrometric analysis, as well as loading control in Western blots. In the reaction, an intense, red - fluorescent enamine forms. But if the proteins present in a basic medium to which Enaminprodukt is deprotonated and loses its fluorescent properties.

Synthetic version

In addition to the natural variation of the fungus of the dye exists now as a synthetic product. With respect to the coloring properties of proteins, there are no differences between the natural and the synthetic variant.