Gene knockin

The knock-in (including gene knock - in, Eng. For, into knock ') referred to in genetics methods for the targeted insertion of a transgene, ie a previously non-existent in a genome gene.

Principle

The insertion of the transgene is usually done in a specific locus in the genome of cell lines or mice. Unlike classical production of genetically modified organisms, the insertion is sequence -specific, resulting in less interference of gene expression resulting from insertion into transcriptionally active regions of the DNA. Through a gene knockin genes can be specifically inserted or inactivated by targeted insertions and then activated by removal of the insertion by a recombinase again. By gene knockin, genes can be inactivated by insertion into promoters, which is used to study the functions of unknown genes or as a model for genetic defect -based diseases.