HEPES
- 4 - (2 -hydroxyethyl) - 1 - piperazineethanesulfonic acid
- N-(2 -hydroxyethyl ) piperazine -N'-( 2-ethanesulfonic acid )
Colorless crystals
Fixed
210-215 ° C
- 7.77 (4 ° C)
- 7.48 (25 ° C)
- 7.39 (37 ° C)
1350 g · l-1 in water (20 ° C)
2000 mg · kg -1 ( LD50, rat, oral)
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HEPES is a short name for 2 - (4 - (2- hydroxyethyl) - 1 - piperazinyl) ethanesulfonic acid, a buffer substance.
Chemical Properties
HEPES is used for biochemical, molecular biological and microbiological purposes as a buffer substance. At an acid constant of pK a = 7.55 (at 20 ° C) it has a good buffer capacity between pH 6.8 to 8.2. The temperature dependence is ΔpKs / T = -0.014 K -1.