Isoschizomer

Isoschizomers as pairs or groups of restriction enzymes referred to in the molecular, which specifically recognize the same nucleotide sequence and cleave in the same way.

5'- GCATGC -3 ' 3'- CGTACG -5 ' 5'- GCATG C-3 ' 3' -C GTACG -5 ' 5 'overhang of four bases ( sticky ends ) PaeI Pseudomonas aeruginosa BBUI Bacillus circulans Other examples of Isoschizomerenpaare are AvaII and Sini, CfoI and HhaI, HpaII and MspI. Apart from the equality of the restriction sequence exist between isoschizomers often no major similarities. These enzymes are isolated from different strains of bacteria and often make different demands on an optimal restriction digestion.