Pfu DNA polymerase

  • CAS Number: 9012-90-2

Pfu polymerase is an enzyme from the thermophilic archaebacterium Pyrococcus furiosus. It is a thermostable DNA polymerase with a proof-reading (English proof-reading ) function that is used in the biochemical laboratory work in the polymerase chain reaction ( PCR).

The Pfu polymerase breaks when reaching erroneous uracil in DNA from the chain extension and, through this readahead function that fewer defective DNA clones are synthesized. The Pfu comes in molecular biology, mainly in those PCR reactions used, must deliver the exact sequence DNA amplicons. For other operations, the PCR much cheaper and faster, but less accurate Taq polymerase is usually sufficient. The Pfu has also compared to the Taq has the advantage that it produces blunt ends so-called blunt ends and no 3'-overhangs attaches from Adenosylresten. The synthesized PCR products are distinguished by blunt ends, which may be for the cloning of advantage.

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