RAPD

RAPD ( ie rapid ) is the abbreviation for. randomly amplified polymorphic DNA, amplified polymorphic DNA randomly German. RAPD is a special form of the polymerase chain reaction. The method was first in 1990 by Williams et al. described.

RAPD primers in short with a length of 8-12 nucleotides can be used that have been randomly generated. From genomic DNA, then only DNA sequence regions that are enclosed by the sequence of the primer reproduce. With appropriate primers result in the individual electrophoresis banding patterns, which make it possible to compare the genetic material of different living things without knowing them in detail. So there are no complicated sequencing required. In practice, the method for studying the phylogenetic relationship of animal and plant species used.

Example

Primer sequence complementary sequence

Source

672550
de