Recombineering

Recombineering ( English by recombinogenic engineering) denotes a molecular biological method for the manipulation of DNA based on homologous recombination in Escherichia coli, which is known under the synonym Red / ET recombination.

Recombineering based on homologous recombination, which is mediated by proteins derived from bacteriophages into Escherichia coli. Two homologous systems are known. The RecE / RecT of the Rac prophage and the red operon consisting of Redγ, Redβ and Redα bacteriophage λ. Both systems allow for the efficient and accurate replacement of freely selectable DNA segments between two different DNA molecules. The red- recombination is most broadly used. The exchange of the target DNA by two homologous flanking fragment ( similar or identical ) sections with a length of 30-50 bp

Benefits

Recombineering can be viewed as an evolution of the conventional cloning, which works independently of restriction enzymes by the use of homologous recombination. Therefore, this method is particularly suitable for the manipulation of large vectors ( > 100 Kb ) such as Bacterial Artificial Chromosomes ( BACs ). Recombineering allows the desired modifications without additional DNA fragments to leave.