TBE buffer
TBE buffer is the acronym for Tris-borate - EDTA buffer according to one of its components
- TRIS (Tris (hydroxymethyl ) aminomethane )
- Borate ( boric acid anion ) and
- EDTA ( ethylenediaminetetraacetic acid)
Named buffer mixture. TBE buffer, inter alia, used in agarose gel electrophoresis. The concentration of the buffer used by the laboratory varied to the laboratory. As a rule, come 0.5X TBE or 1X concentrated for use.
Production
For the production of TBE - buffer ( 1X ), pH 8.0, for example, be
With distilled ( demineralized ) water filled up to 1000 ml.
Alternative
Alternatives include, for example, the TAE buffer, and the LB buffer.