Transcriptome

The transcriptome is the sum of all transcribed at a given time in a cell, that is, the DNA rewritten into RNA genes, ie the entirety of all produced in a cell RNA molecules.

Properties

The concept is similar to the proteome, the entirety of the present to a certain time in a cell proteins. But not every present after transcription RNA, such as rRNA or RNA of ribonucleoproteins, in a protein translated ( translated ) is and mRNAs can still be processed, proteome and transcriptome of a cell are not identical.

For the analysis of the transcriptome is resorted generally to methods such as RT- PCR with degenerate primers, followed by DNA microarray or DNA sequencing in high throughput (RNA -Seq ). RNA -protein interactions can be studied with methods such as RIP - chip, CLIP -Seq, PAR -CLIP or iCLIP. Another method in which the activity of unknown genes can be observed, is the so-called serial analysis of gene expression ( SAGE ) and Super announcement.

The term refers to the study of the transcriptome the mRNA expression of a cell. The term is an analogy to the term proteomics and synonymous for gene expression analysis.