Widal test

The Gruber - Widal reaction, named after the hygienist Max von Gruber (1853-1927) and the French pathologist Fernand Widal (1862-1929), the detection of specific antibodies using antigens serves to known bacteria. By determining the titre of the Gruber - Widal reaction allows a conclusion as to the concentration of these antibodies in the blood serum of a patient.

Implementation

Blood serum is diluted in ascending concentrations in physiological saline solution, combined with a suspension of killed bacteria and incubated for 24 hours at 37 ° C, the agglutinate of antibody and antigen-bearing bacteria precipitates in the form of a white turbidity. The greatest dilution with precipitation indicates the titer. The titer can be read with a Agglutinoskop. Important for assessing whether an acute infection is present, is other than the amount of the progress of the titre: For an acute infection of a rise in titer is within seven days of a condition. The Gruber - Widal reaction is a diagnosis, for example of salmonellosis, Escherichia coli infections and dysentery. In modified form, it is also used in the detection of brucellosis as Widal -Wright reaction.

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