Zeocin

• Zeocin
• N1 -[4 - [( aminoiminomethyl ) amino] butyl] -7,8- dihydro- bleomycinamid

• C55H86N20O21S2
• C55H86N20O21S2Cu · HCl ( Kupferchelatkomplex )

Bluish powder (as Kupferchelatkomplex )

Phleomycin

Attention

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Phleomycin D1 is a copper- chelating glycopeptide antibiotic. It is mainly known under the trade name Zeocin, which mainly includes phleomycin D1. It belongs to the family of bleomycins and is also isolated from a mutant of Streptomyces verticillus. The mechanism of action is not yet clearly elucidated but Zeocin has a wide range of target organisms ( prokaryotes and eukaryotes ).

Properties

Due to the copper complex crystals are formed bluish tint. Phleomycin D1 loses its activity in the acidic and basic region as well as in the presence of oxidative reagents.

Use

Phleomycin D1 is mainly used in molecular biology research. The big advantage is that it is, for example, acts not only against bacteria or yeasts, but against both. This allows the use of vectors for cloning, which are suitable for bacteria and yeast, and then only one resistance gene for the selection is necessary.

Operation

As previously mentioned the mode of action is not yet fully understood. However, it is assumed that phleomycin D1 enters the cell and the DNA intercalation. Then there is the metal ion (copper ) to a cleavage of the DNA. The genetic material of the cell is damaged and it is no longer viable.

Operation of the resistance

The resistance gene Sh ble enables the cell to express a 14 kDa protein which binds specifically to Phelomycin D1. This antibiotic is inhibited in its action and effect.