3D cell culture

3D cell culture refers to the cultivation of cells in a micro- structured three-dimensional cell culture under in vitro conditions. Just as the organs in the body take a spatial orientation, this will probably be implemented in cell cultures. In contrast to tissue engineering 3D cell culture is not intended primarily for the production of bodies.

Properties

The term 3D cell culture includes, among others, the cultivation of cells in three-dimensional hydrogels formed from layers of plastic or scaffold protein (English scaffold, scaffold '). The cells lie in the cellular aggregates with a freely definable diameter ( spheroids ) ago. Also, the culture of tissue sections is part of the 3D cell culture. The 3D cell culture tried to consider organ-specific conditions and largely engages mostly in the processing of plastics, but also natural products back that have already proven to be applicable for cell cultures.

Applications

The 3D cell culture is used in pharmacology for partial replacement of animals and 2D cell cultures. In addition, it is more suitable for high-throughput screening as experimental animals, does without, however, to the study of the interactions between the organs in an organism. There is evidence that an organ- like behavior of the cell culture leads to more meaningful results if the mode of action of a new drug to be tested and thereby draw conclusions regarding its pharmacokinetics and pharmacodynamics must be drawn. There are various 3D organ models for each entry and the place of action of the substance, such as skin, liver, pancreas or lungs. Other areas of application are the study of stem cells or tumor research In addition, 3D cell cultures are used in the research field of regenerative medicine to produce, for example implants for cartilage or to facilitate wound healing processes. Further 3D cell cultures for the production of in vitro meat may be used.

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