ADP ribosylation factor

Adenosyl- ribosylation factor (ARF ) ( and ADP - ribosylation ) is a protein belonging to the family of small GTPases. It exists in several subversions ( ARF1 -6), whose molecular mass is in the range of 21 kDa.

Was discovered an important function of ARF1 in the study of cholera infection. It serves as a cofactor for the permanent ADP-ribosylation of the adenylate cyclase -stimulating subunit of a trimeric G protein., The resulting increase of cAMP in the intestinal cells diarrhea typical of cholera resulted.

Another important function of ARF1 is the switch function in the genesis of intracellular protein transport vesicles at the Golgi apparatus. There will cycle between a GTP - and GDP-bound form and recruits required for the vesicle coat proteins, which are assembled and subsequently pinch off the vesicle from the membrane. The cyclic nucleotide change is by nucleotide exchange ( GEFs ) and GTPase - activating proteins catalyzed ( GAPs ).

Like all GTPases also has a central ARF Mg2 ion, which is necessary for the complexation of the nucleotide GTP.

G-Protein Cofactor (biochemistry) Intestine Magnesium
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