Keothavong was born in 1983 in Hackney, London. Her parents fled in the 1970s from the war-torn Laos. Early on, encouraged the father, a bank employee, his children, to learn to play tennis. Anne's brother James Keothavong is seen as a Chair Umpire in tennis tournaments.
Keothavong, who has a powerful forehand from the baseline and a good first serve, prefers to play on hard courts.
On the ITF Women's Circuit, she won 20 singles and 8 doubles titles. At the Wimbledon Championships, she went in 2000 for the first time at a Grand Slam tournament at the start, but failed in the second round of qualifying. Her best performance came in 2008 her at the U.S. Open when she moved there in the third round.
In the Bank of the West Classic 2009 in Stanford itself Keothavong badly injured his left knee as she bounced into a double match in an attempt to Walk a drop shot, against a net post. Here cruciate ligament and meniscus were injured, the same injury she had sustained in his right knee in 2004. After a six month break, she played her first tennis match in February 2010 in the Fed Cup.
On July 24, 2013 Keothavong announced their careers end. From the end of 2013 she wanted to work as a reporter for BT sport.