Assay

The word assay ( German: test sample) comes from the English -speaking world and is there a general examination for the detection of certain substances (eg metals). In the German language assay referred mainly in laboratory medicine and pharmaceutical research a standardized course of the reaction for the detection of a substance with a specific method. In particular, this refers to molecular biological methods.

There is no special feature that distinguishes an assay over other detection methods, at best that the occurrence of the name indicates the origin of the name or the method from the English-speaking world. In many cases, the assay was replaced with other words (for example, Bradford assay ).

Examples

Detection of DNA and their binding to proteins

  • DNase footprinting assay
  • Electrophoretic mobility shift assay

Detection of RNA

  • Nuclear run-on

The detection of protein

  • BCA assay, see bicinchoninic
  • Bradford assay
  • Lowry assay
  • Secretion assay

Detection of viruses

  • Plaque assay
  • Radioimmunoassay (RIA)
  • Enzyme -linked fluorescent assay ( ELFA )
  • The electrochemical luminescence immunoassay ( ECLIA )
Bradford protein assay Bioassay
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