Beta-glucuronidase

  • OMIM: 253220
  • UniProt: P08236
  • MGI: 95872
  • CAS Number: 9001-45-0

β -glucuronidase ( GUSB ) is the name for enzymes that cleave β - glucuronides, which are glycosides, which are derived from glucuronic acid. This reaction step is essential for animals and some bacteria in the degradation of heme substances, chondroitin sulfate and keratan sulfate. The enzyme is found in the lysosomes of liver, spleen and tonsils. Defects in GUSB gene in humans can cause mucopolysaccharidosis type 7 and play a role in fetal hydrops.

Use

It finds applications in biotechnology as a reporter gene. Their activity can be detected by various methods:

  • β -glucuronidase hydrolyzed X -Gluc, which a blue dye;
  • 4- methylumbelliferyl- β -D -glucuronide (MUG ) is added to 4-methylumbelliferone (MU ) is hydrolyzed, which is detected by its fluorescence. Here, this fluorescence can be concluded that the turnover rate of the β -glucuronidase on the intensity.
  • β -glucuronidase cleaves p-nitrophenyl- β -D -glucuronide in glucuronic acid and p- nitrophenol. p- nitrophenol dissociated by a base in p- nitrophenolate, which is colored yellow.
Protein isoform Human Genome Organisation Mouse Genome Informatics CAS registry number Hydrolysis Organism Hydrops fetalis X-Gluc Mug (disambiguation) Hymecromone
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