The bioanalysis deals with the analytical methods in the life sciences: biochemistry, molecular biology, molecular genetics, cell and developmental biology and medicine.
In a narrow sense bioanalysis examined biological macromolecules and their changes, that is, proteins, DNA, RNA, carbohydrates, and lipids. In a broader sense, low molecular weight substrates and products of metabolism to be added of biological systems.
As the " tools " are in bioanalysis devices uses the physio- chemical parameters with suitable sensors or biosensors convert into electrical signals which can then be qualitatively and quantitatively detected and recorded by the experimenter. The case registered data can be very complex and are analyzed using bioinformatics.
In addition to the conventional high performance liquid chromatography (HPLC) with UV or fluorescence detector is also increasingly the combination with a mass spectrometer (MS ) is used. The HPLC-MS/MS-Kombination (serial "Tandem" ) sometimes allows the selective detection of concentrations ( for example, in blood plasma) into the area of pg per ml
Physical separation processes, as presented, for example, for preparative processing of biological products, mostly used for the isolation of the desired bioproduct from the mass of impurities and with the least possible error to allow the qualitative and quantitative determination of the bioproduct. Often the organic product must be modified by a chemical modification with or without biocatalysis that a detection or separation is possible. In any case, bioanalysis due to the higher complexity in most cases more expensive in terms of the accuracy and reproducibility as a classical physico- chemical analysis.
In the pharmaceutical research (future drugs ), metabolites and antibodies are quantified in body fluids and tissues to determine which are important for the pharmacokinetics LADME parameters by means of Bioanalysis agents. Furthermore, the results of bioanalysis for determining the toxicokinetics be used. This work will i.a. carried out under the quality system ' Good Laboratory Practice ' (GLP ), thus subject to strict legal regulations.
For sample management and tracking i a a Laboratory Information Management System (LIMS) is used, it must be that for GLP studies requiring fully qualified.