Bromodeoxyuridine

5 -bromo-2 -deoxyuridine (BrdU)

192-193 ° C

Very poor in water ( 970 mg · l-1 at 25 ° C)

Risk

8400 mg · kg -1 ( LD50, rat, oral)

Template: Infobox chemical / molecular formula search available

Bromodeoxyuridine (BrdU ) is a chemical analog of the nucleoside thymidine or deoxyuridine. It is used for laboratory diagnostic marker of proliferating cells in vital tissues. It is formed by connection of deoxyribose to the nucleobase 5 - bromouracil.

BrdU can be absorbed by the cell and will be incorporated during the S phase of the cell cycle into the newly synthesized DNA in the place of a phosphorylated form of the nucleotide deoxythymidine triphosphate ( dTTP ). Specific antibody to BrdU are used to detect a DNA synthesis was carried out by immunohistochemistry. For the binding of the antibody to BrdU denaturation of the DNA is necessary, which is usually carried out with acid or heat.

BrdU as can replace thymidine during DNA replication, it is capable of eliciting mutations. It is therefore potentially harmful to health.