Bromsulphthalein
- BSP
- Bromosulfophthalein
- Sulfobromphthalein
- 3,4,5,6, - Tetrabromphenolphthalein - di -sulfonic acid
- 3,3 ' - ( 4,5,6,7 -tetrabromo -3-oxo -1 ( 3H)- isobenzo - furanyliden ) bis [ 6- hydroxybenzolsulfon ] acid
- 297-83-6
- 71-67-0 (disodium salt )
V04CE02
Diagnostic, liver function
- 794.03 g.mol -1
- 837.997 g · mol -1 ( disodium salt)
Risk
Template: Infobox chemical / molecular formula search available
Bromosulfophthalein also Bromsulfalein or BSP is a diagnostic to check liver function. It is a brominated sulfonic acid derivative of phenolphthalein.
Properties
BSP is analogous to a phenolphthalein purple in the alkaline colorless in an acidic dye. Its lambda max is at 577 nm
Use
BSP is used in Bromsulfaleintest to investigate the excretory liver function, which was established in 1938. Today, however, used rather the transaminases. When Bromsulphthalein clearance test, the substance is administered intravenously. In the blood, it binds to albumin. Through the liver it is excreted in the bile. The dye concentration in the blood serum is a measure of the liver function. The absorbance is measured at a wavelength of 552 nm, because the interfering hemoglobin then in acidic equally absorbed as in the alkaline range.
Pharmacological properties
BSP is primary active ATP-consuming by transporter proteins ( Bile Salt Export Pump ( BSEP ) and multidrug resistance -related protein ( MRP2 ) ) eliminated in the bile formation. In the context of distributing, by infusion continuously fed radiolabeled 35S BSP in dogs has been found that this and its metabolites sporadically occurred also in the urine of the treated animals; the majority of the administered substance was found with 48-75 % in the liver. Organs, which in comparison to the plasma had high concentrations were stomach, intestines, pancreas, intercostal muscle, and especially the kidneys. Wherein there is no correlation between the concentration in the kidneys in the urine and the appearance was observed.
Proof
The difference in color intensity at the transition from the alkaline to acidic region is proportional to the concentration. The determination can therefore be photometrically.