Carboxyfluorescein

• 3 ', 6' -dihydroxy-1 - oxospiro [2 -benzofuran- 3,9 '- xanthen ]-5 -carboxylic acid ( IUPAC)
• 3 ', 6'- dihydroxy-3- oxospiro ( isobenzofuran- 1 (3H), 9'- ( 9H) xanthen )-6 -carboxylic acid
• 6-FAM
• FLUOS

Red solid

Fixed

> 300 ° C

Soluble in DMF or DMSO

Attention

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6 -carboxyfluorescein (6- FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm It is used in the sequencing of nucleic acids and in the selection of nucleotides.

The frequently encountered FAM label is not correct, but reflects the fact that the commercially available product of the two isomers of 5 -FAM and 6 -FAM is.

Use

In the quantitative analysis of nucleic acids by real- time PCR, the phosphoramidite derivative 6-FAM -phosphoramidite is used. With this addition to the introduction of the hydroxyl groups of Phosphoramiditrestes 6-FAM at the positions 3 'and 6' with dimethylpropanoic acid are esterified. What the absorption and emission wavelengths of 6- FAM -phosphoramidite and 6- carboxyfluorescein differ.

Another known derivative of 6- carboxyfluorescein succinimidyl ester is His, which is the same application, such as the phosphoramidite, contrary to this, but such as 6- FAM has an absorption wavelength of 495 nm and an emission wavelength of 517 nm.