Cycloheximide

• 4 - [( 2R ) -2 - [ (1S, 3S, 5S) - 3,5-dimethyl- 2- oxocyclohexyl ] - 2-hydroxyethyl ] piperidin- 2 ,6-dione
• Actidion
• Naramycin A
• CHX

Colorless crystals

Fixed

119-121 ° C

Poorly in water ( 21 g · l-1 at 20 ° C)

Risk

2 mg · kg -1 ( LD50, rat, oral)

Template: Infobox chemical / molecular formula search available

Cycloheximide is an antibiotic derived from Streptomyces ( Streptomyces griseus ) is produced.

It acts as a translational inhibitor in eukaryotes and performs by blocking protein synthesis at the ribosome to inhibit the growth and death of eukaryotic cells. It is a classic translational inhibitors. Mitochondrial and chloroplast protein synthesis in eukaryotic cells is also disrupted by very high concentrations of cycloheximide, which is why this antibiotic may be used for the selection of transgenic plants ( eukaryote ). Cycloheximide hardly finds use as an antibiotic, as it at high doses inhibits the mitochondrial protein translation in man.

Use

• Selection of prokaryotic cells, suppression of eukaryotic contaminants
• Selection of resistant cells in transformation and cloning experiments in eukaryotes (fungi )
• Controlled inhibition of protein synthesis in cell cultures and detection of short-lived proteins
• Induction of apoptosis and Others