Dithiothreitol

• Cleland's reagent
• 1,4- dimercapto -2 ,3-butanediol
• Butane -2 ,3 -diol -1 ,4 -dithiol

• 3483-12-3 (DL -threo )
• 6892-68-8 ( erythro )
• 27565-41-9 (DL- ( -) -threo- form)
• 16096-97-2 (L -threo )
• 7634-42-6 (unspecified)

White solid with an unpleasant odor

Fixed

• 40-43 ° C ( DL -threo )
• 50-52 ° C (L -threo )
• 82-83 ° C ( erythro )

Very well in water (1500 g · l-1 at 20 ° C)

Attention

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Dithiothreitol (DTT ) is a chemical compound which is also known as Cleland's reagent. It is derived formally from the sugar alcohol threitol or threitol from that belongs to the structure type of Threose. It comes in two stereoisomers ago: The ( 2S, 3S)- form (1) is derived from the D- Threose, the (2R, 3R )-form ( 2) of the L- Threose.

DTT is used in the protein biochemistry. It conserves proteins from the cell inside in its functional form, by preventing the oxidation of sulfhydryl groups ( SH ) to disulfide bonds by atmospheric oxygen. On the other hand, the folding of proteins whose structure is stabilized by disulfide bridges, destroy by their reduction. It is an important reagent in the sample buffer for SDS-PAGE and Western blotting.

Oxidation leading to the formation of an intramolecular disulfide bond which forms a six-membered ring. This is energetically strongly favored the oxidized form.