EGTA (chemical)

  • EGTA
  • Ethylenebis ( oxyethylenenitrilo ) -tetraacetic acid
  • 1,2-bis -(2- aminoethoxy ) ethane -N, N, N ', N'-tetraacetic acid
  • Complexone VI
  • Titriplex VI

White powder

Fixed

241 ° C ( decomposition)

3587 mg · kg -1 ( LD50, rat, oral)

Template: Infobox chemical / molecular formula search available

Ethylene glycol bis ( aminoethyl ether )-N, N, N ', N'-tetraacetic acid, abbreviated EGTA, a polyamino carboxylic acid, which is used in chemistry and the life sciences, as the more common related EDTA as chelating agent; However, with a much higher affinity for calcium than for magnesium ions. Therefore, EGTA is useful to prepare buffer solutions, adjust the milieu in plasma of living cells.

The pK a value for the binding of calcium ions by EGTA tetrabasischem is 11.0, but the protonated forms will not contribute significantly to binding. At pH 7, the apparent pKa value therefore falls to 6.91.

EGTA was experimentally used for the treatment of animals with cerium poisoning and for the isolation of thorium from the mineral monazite. Furthermore, EGTA part in elution buffers for protein isolation technology TAP ( Tandem Affinity Purification ). Recombinant fusion proteins are bound by calmodulin beads and released by added EGTA.

In dentistry EGTA is used for removing deposits in the tooth root canal.

CAS registry number PubChem
297751
de