Epsilometertest

E test is the short notation for Epsilometertest. It is a method to determine in a simple way to microbiology, the minimum inhibitory concentration (MIC ) of an antibiotic for a bacterium to be examined.

The test consists of a plastic strip, which is provided on one side with an antibiotic. The concentration of this antibiotic rises from the lower to the upper end of the strip down exponentially in stages to what is marked on the strip with graduations. The strip is placed on a bacteria culture that is streaked as a bacterial lawn. This is then generally incubated at 37 ° C for 24 hours. In this case, the antibiotic to diffuse into the agar and inhibit in a specific zone of bacterial growth. Based on this zone of inhibition and the graduations can be read, which is the minimum inhibitory concentration required to inhibit an effective implementation of what determines the amount of medication to the patient.

The test provides compared to the simple antibiogram more accurate picture of the resistance behavior of a bacterium.

The E- test there is to investigate the resistance behavior of fungi also available in versions with antifungal agents. The operation is the same.

Swell

  • Baker CN: The E- Test and Campylobacter jejuni. Diagn Microbiol Infect Dis. July 1992, 15 ( 5) :469 -72. PMID 1643827
  • Rosser, SJ, Alfa MJ, Hoban S, Kennedy J, Harding GK: E test versus agar dilution for antimicrobial susceptibility testing of viridans group streptococci. J Clin Microbiol. 1999 Jan; 37 ( 1) :26 - 30th PMID 9854058
  • Product page Biomérieux: http://www.biomerieux.de/servlet/srt/bio/germany/dynPage?doc=GRM_CLN_PRD_G_PRD_CLN_64
  • Microbiological test method
Antifungal medication
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