FlAsH-EDT2

4 ', 5' -bis ( 1,3,2- dithioarsolan -2-yl ) fluorescein

Fixed

Slightly soluble in water

Risk

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Fluorescein arsenical helix binder, FlAsH EDT2 short, is a derived from the fluorescent dye fluorescein arsenical chemical compound that can bind to peptides and proteins selectively to certain structures (so-called Tetracysteinsequenzen ). After binding to its target structure and an excitation with short-wavelength light shows FlAsH fluorescence. FlAsH EDT2 is therefore suitable as a protein tag for the selective labeling and identification of proteins. As said dye as a fluorophore for Förster resonance energy transfer ( FRET) is used.

Biochemistry

The preferred bonding structure for Flash EDT2 Tetracysteinsequenz is constructed from four Cysteinbausteinen and a spacer consisting of two amino acids in the form CCXXCC. Lower affinity FlAsH EDT2 to other structures, such as CXXC.

Synthesis

FlAsH EDT2 can be ', 5' -bis ( acetoxymercuri ) fluorescein synthesized in a two-step synthesis of 4. For this purpose, 4 ', 5' -bis ( acetoxymercuri ) fluorescein with a molar excess of arsenic (III ) chloride and diisopropylethylamine in N-methylpyrrolidone reacted under palladium catalysis. The reaction product is then reacted with an excess of 1,2- ethanedithiol to FlAsH EDT2.