Lanosterol 14 alpha-demethylase

The enzyme lanosterol demethylase (also: CYP51A1 ) catalyzes the dehydrogenative demethylation of lanosterol to 4,4-dimethyl- 5α -cholesta -8 ,14,24 - trien- 3β -ol. This chemical reaction is an intermediate step in the cholesterol biosynthesis which takes place in eukaryotes as well as in some bacteria. It is a monooxygenase by cytochrome P450 type, ie with heme as a cofactor. CYP51A1 is produced by humans in many tissue types.

Catalyzed reaction

3 O2 3 NADPH formate 4 H2O 3 NADP

In several oxidation steps, the 14 -methyl group is removed and the Lanosterins creates a 14,15- double bond, wherein the formate is cleaved. Other 14 - methyl steroids ( Eburicol, Norlanosterin, Obtusifoliol ) are accepted as a substrate.

Ketoconazole Protein isoform Human Genome Organisation Cofactor (biochemistry)
210835
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