LB buffer

Lithium borate buffer ( LB buffer) is a buffer that is used in biochemistry and molecular biology in the course of agarose gel electrophoresis for the separation of nucleic acids such as DNA or RNA.

Properties

The LB buffer is composed of lithium hydroxide and boric acid, and, in comparison with the alternative buffer ( TAE buffer, TBE buffer, sodium borate buffer), a lower ionic strength, and therefore have a lower electrical conductivity. This allows a higher applied voltage and therefore a higher rate of migration of nucleic acids without heating the agarose gel. This shortens the time required for electrophoretic separation. The rights of commercial use of the LB buffer in the U.S. are protected by U.S. patent 7,163,610 of the company Faster Better Media LLC.

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