Luigi Maglione was a seminarian at the Almo Collegio Capranica, he studied at various universities of Rome and a doctorate, both in Catholic theology and in philosophy.
He received on 25 July 1901, the sacrament of Holy Orders and worked until 1908 as a vicar in the diocese of Naples. By the way, he ran further studies and entered 1908 in the diplomatic service of the Vatican, where he worked in the Vatican Secretariat of State until 1918. In the years 1915-1918 he taught also at the Pontifical Ecclesiastical Academy. 1918 Pope Benedict XV appointed him. for preliminary Pontifical Representatives the League of Nations and to the Messenger in Switzerland.
1920 Luigi Maglione received the appointment as Titular Archbishop of Caesarea in Palestine and Apostolic Nuncio for Switzerland. The bishop He was ordained by Cardinal Pietro Gasparri. From 1926 to 1935 he was Apostolic Nuncio to France. 1935 took him Pope Pius XI. on a cardinal priest with the titular church of Santa Pudenziana in the College of Cardinals and in 1938 he was entrusted with the office of Prefect of Congregation of the Council (now the Congregation for the Clergy ).
A few days after the election of Pope Pius XII. was appointed in March 1939 to the Cardinal Secretary of State Luigi Maglione of this. From 1939 headed Luigi Maglione as Magnus Cancellarius the Pontifical Institute of Christian Archaeology. He died on 22 August 1944 in Casoria, where he was also buried.