Murashige and Skoog medium

The Murashige and Skoog medium - also MSO or MS0 (MS- zero) - is a plant growing medium, which is used in laboratories for the cultivation of plant tissue cultures. It was developed by Toshio Murashige and Folke K. Skoog looking for a new Pflanzenwachstumsregulatur. It is the most common medium in plant tissue culture experiments.

Skoogs Murashige PhD student should discover a growth hormone in tobacco juice, but this did not succeed. Instead, analyzes of wrung tobacco and tobacco ashed higher concentrations of certain minerals in plant tissues than previously thought. After some experimentation it has been shown that the variation of the levels of these nutrients, especially nitrogen, produced an increased growth.

Ingredients

Macronutrients

Ammonium nitrate ( NH4NO3 ), boric acid ( H3BO3 ), calcium chloride (CaCl2 · 2H2O), cobalt chloride ( CoCl2 · 6H2O ), magnesium sulfate ( MgSO4 · 7H2O ), copper sulfate ( CuSO4 · 5H2O), potassium dihydrogen phosphate ( KH2PO4 ), iron ( II ) sulfate ( FeSO4 · 7H2O, potassium nitrate ( KNO 3 ), manganese sulfate ( MnSO4 · 4H2O ), potassium iodide (KI ), sodium ( Na2MoO4 · 2H2O ), zinc sulfate ( ZnSO4 · 7H2O ), Na2EDTA · 2H2O

Organic additives

Often added: inositol, niacin, pyridoxine HCl, thiamine · HCl, IES, kinetin, glycine, Edamin S, sucrose, agar