Neoschizomer

Neoschizomere as pairs or groups of restriction enzymes referred to in the molecular, which specifically recognize the same nucleotide sequence, but these columns in different ways.

5'- CCCGGG -3 ' 3'- GGGCCC -5 ' 5'- CCC GGG -3 ' 3'- GGG CCC -5 ' no overhang ( blunt ends ) XmaI Xanthomonas malvacearum 5'- CCGGG C -3 ' 3'- GGGCC C-5 ' 3 'overhang of four bases ( sticky ends ) Other examples of Neoschizomerenpaare are KpnI and Acc65I, SacI and EcoICRI, AatI and ZraI. Apart from the similarity of the recognition sequence existing between Neoschizomeren often no significant similarity in amino acid sequence and structure. These enzymes are isolated from different strains of bacteria and often make different demands for optimal restriction digestion.