pUC19

PUC19 and pUC18 are man-made, bacterial plasmids belonging to the vectors for cloning and expression of proteins in Escherichia coli, the most commonly used. Thus, they have a great importance in biological research and genetic engineering. Their special advantage is a particularly high number of copies per bacterial cell ( so-called high copy number plasmids ), so easily get a large amount of plasmid can be isolated.

Properties

Developmentally, there are derivatives of pBR322 plasmid. The pUC plasmids using the modified origin of replication of pBR322 plasmid. The ori was reduced and it lacks the coding region for the ROM / ROP protein. By knockout of ROP copy number ColEl plasmids can generally be increased as long as the cultivation temperature is> 30 ° C .. In addition, the modified ori a point mutation G → A at position 112 of the RNA II. This results in the increased copy number of these vectors.

They are relatively small with 2686 bp, and they may have additional DNA fragments are inserted at specific interfaces. Transcription of the inserted genes in E. coli can be induced by the built- lac operon ( N-terminal fragment ) specifically initiated with IPTG. For selection of pUC -positive bacteria after insertion of the plasmid into an appropriate bacterial strain ( transformation ) is an existing in the pUC plasmids, ampicillin resistance gene ( amp R ) and the blue - white selection. The two pUC plasmids differ only in the inverse orientation of the multiple cloning sites (MCS ), consisting of a succession of different restriction sites.

The name of plasmids derived from the University of California (UC ), in which these plasmids (P) were constructed.

Swell

664473
de