RIP-Chip

RIP- chip (English RNA immunoprecipitation chip, RNA - immunoprecipitation microarray " ) is a method for the detection of the biochemistry of RNA -protein interactions. RIP- chip is a combination of immunoprecipitation of an RNA -binding protein with a RT-PCR and microarray, and is used for examining the various bound RNA of such a protein.

Principle

By immunoprecipitation of a known, most recombinant protein -specific binding RNA sequences can be enriched and purified from an RNA solution. This RNA is then separated from the protein and transcribed by RT-PCR in cDNA. This generated cDNA is used for hybridization with DNA on the microarray.

The RIP - chip has similarities to the ChIP -on-chip, which characterizes bound with similar principle DNA sequences of DNA-binding proteins. The CLIP -Seq, PAR -CLIP and iCLIP are an alternative method for RIP - chip with DNA sequencing in high-throughput instead of the microarrays.

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