From 1996 to 2002, Botero went for the Kelme -Costa Blanca team for which he scored his greatest successes. Like most of his teammates was Botero first as a climbing specialist, but since 2002 he appeared rather as a strong time trialist. At the Tour de France in 2000, he won a stage for the first time, won the mountain jersey of the Tour and finished second in the overall standings to fifth place. Two years later he even reached the fourth place, beating surprising Lance Armstrong in the first time trial of the Tour 2002. Overall, he won for Kelme three stages of the Tour de France.
Also in 2002, he experienced perhaps his greatest triumph when he became world champion time trial at the Road World Championships in Zolder, Belgium.
In the years 2003 and 2004, Botero drove for the team Telekom and T - Mobile team. In both years, he succeeded against all expectations the team management not to build on past successes, as he was often plagued by colds and injury problems. After moving to the Swiss team Phonak in 2005, he returned to winning ways and was the beginning of May of the year the Tour de Romandie - thanks mainly to a sovereign victory in the final time trial - win. In the eleventh stage of the Tour de France 2005 from Courchevel to Briancon he made a strong impression that he avulsion along with Alexander Vinokourov at the mountain rise before the peak group to Lance Armstrong and came only by the stronger commencement of the Kazakhs in the target sprint to second place. In 2006, he starts at the Clasica Alcobendas in early May in the season. In his first Pro Tour race in that year, the Tour of Catalonia from 15 to 21 May, he finished second.
A little later the name of Santiago Botero in the wake of the Spanish doping scandal was named as one of the doctor Fuentes customers. Therefore Phonak pulled him immediately from the team that the Dauphine Libere should deny back. It was also locked out of his team as well as team-mate Gutierrez for the Tour de France.
In early November the investigation due to lack of evidence from the Colombian Cycling Federation has been set. A few weeks later Botero signed a contract with the Colombian team UNE- Orbitel a new contract after the Phonak team disbanded at end of season. In 2008 he drove for the U.S. Continental team rock racing.
In July 2010, Botero ended his cycling career.
- 2002 Classique des Alpes
- 2002 world champion - time trial
- 2005 Tour de Romandie
- 2007, two stages, Prolog and Rating Colombia Tour
- 2007 Colombian national time trial champion
- 2008 Redlands Bicycle Classic Stage (USA)
- 2008 Vuelta a Colombia stage
- 2009 Colombian national time trial champion
- 2009 Vuelta a Colombia stage
- 3 Stages Tour de France
- 3 stages Vuelta a España