SDD-AGE

SDD -AGE ( ENGL. Semi- denaturing detergent agarose gel electrophoresis, halbdenaturierende detergenzvermittelte agarose gel electrophoresis ') is a biochemical method in which SDS-resistant protein complexes, such as in amyloidosis or prion diseases, by gel electrophoresis in an agarose gel be separated and detected.

Principle

SDS-resistant proteins are complex composites of several proteins that ( w / v ) non- denatured at a concentration of 2% SDS at room temperature. Their molecular weight is generally between 200 and 4000 kilo Daltons kilodaltons why an agarose gel is used with a relatively large pore diameters. After the electrophoresis is carried out, analogous to the Western blot in a polyacrylamide gel, a protein transfer to a PVDF membrane, and immunostaining of the proteins on the membrane. This Konformationsvarianten the proteins of a protein aggregate may be distinguished.

719626
de