She trained as a projectionist at the Madras Film Institute. In 1980, she received her first film role in Nenjathai Killathey. Suhasini quickly evolved into a theatrical personality, often playing feminist roles, as well as in Swati (1984) and Lawyer Suhasini (1987). They occurred mainly in Malayalam films, often with the Megastar of South Indian film Mammootty on.
Suhasini is the niece of the Tamil film star Kamal Haasan. She is married to the Tamil film director Mani Ratnam since 1989.