Maniatis graduated from the University of Colorado in 1965 a bachelor's degree in biology and chemistry in 1967 and a Masters in Biology. In 1971, he earned a Ph.D. at Vanderbilt University in molecular biology. As a postdoctoral fellow Maniatis worked at Mark Ptashne at Harvard University and at Fred Sanger in Cambridge, England.
1974 Maniatis received a first position as a research assistant at Harvard University in Cambridge, Massachusetts. In 1975 he was a research group leader at Cold Spring Harbor Laboratory on Long Iceland and assistant professor of biochemistry and molecular biology at Harvard University. In 1977 he became associate professor of biology at the California Institute of Technology in Pasadena, California, in 1979 he became a full professor. 1981 went Maniatis Professor of Biochemistry and Molecular Biology Back to Harvard University from 1985 to 1988 as head of the department. In 1995 he became a professor of molecular and cell biology at Harvard.
2010 moved Maniatis to Columbia University in New York City, where he (as of 2012) is Head of the Department of Biochemistry and Molecular Biophysics today.
Maniatis was able to make fundamental contributions to the understanding of the structure and function of deoxyribonucleic acid ( DNA), thus laying the foundations for the development of techniques for artificial recombination of DNA (genetic engineering ) and allowed new insights into the organization of the chromosomes of eukaryotes. His work on the genes of the globin showed the different levels at which there is a regulation of gene expression, thereby laying the basis for the investigation of the molecular causes of human genetic diseases.
Maniatis ' textbook Molecular cloning: a laboratory manual is considered a standard work of genetic engineering.
Recent work dealing with the genetic causes of amyotrophic lateral sclerosis ( ALS).