Weigert's elastic stain
The Elastica staining or Resorcinfuchsinfärbung Weigert (1898 ) is a special histological staining to color isolated illustration of elastic fibers in histological sections.
Färbeprinzip
With the help of offered in excess, the positively charged dye resorcin-fuchsin (or orcein ), it is possible that color due to their dense and seamless package actually bad stainable elastic fibers contrasts represent. By interfacial adsorption, the positively charged dye superposed in the acidic electro- polar and thus negatively charged coating layer of the elastic fibers of ( Elastomucin ). If desired, be used to counter-staining of the other cell components, in particular for representation of the nuclei, nuclear fast red, which has been similar hemalum previously recognized with aluminum sulfate as a mordant.
Reagents
Resorcin-fuchsin contains the basic and positively charged dye Diamantfuchsin or orcein in alcoholic solution.
A related primarily to the nuclear staining dye, which, however, before a mordant such as alum must be buried so that he can fully develop its dyeing effect.