Eagle's minimal essential medium

Dulbecco 's Modified Eagle's medium, DMEM short, is a standard growth medium for cell cultures with a broad applicability for various human and animal cells. It is a variant of Eagle's Minimum Essential Medium ( EMEM ), but contains more nutrients than this. It was named after Renato Dulbecco and Harry Eagle.

Composition

In mg / l:

Inorganic salts:

• CaCl2 (anhydrous): 200.00
• Fe ( NO3) 3 · 9 H2O: 0.10
• KCl: 400.00
• MgSO4 (anhydrous): 97.67
• NaCl: 6400.00
• NaH2PO4 · H2O: 125.00
• NaHCO3: 3700.00

Other components:

• D- Glucose: 4500.00
• Phenol: 15.00
• Sodium pyruvate: 110.00

Amino acids:

• L -Arginine HCl: 84.00
• L- cystine 2HCl: 63.00
• L- Glutamine: 584.00
• Glycine: 30.00
• L- histidine HCl H2O: 42.00
• L- isoleucine: 105.00
• L- leucine: 105.00
• L- lysine HCl: 146.00
• L- Methionine: 30.00
• L- Phenylalanine: 66.00
• L- Serine: 42.00
• L -threonine: 95.00
• L- tryptophan: 16.00
• L- tyrosine 2Na · 2H2O: 104.33
• L- valine: 94.00

Vitamins:

• D -Calcium: 4.00
• Choline chloride: 4.00
• Folic acid 4.00
• I- inositol 7.20
• Niacinamide 4.00
• Riboflavin 0.40
• Thiamine HCl 4.00

• Nutrient