N-Ethylmaleimide

Colorless crystals with a pungent odor

Fixed

0.33 g · cm -3

43-46 ° C

210 ° C.

1 g · l-1 (at 20 ° C)

Risk

  • 25 mg · kg -1 ( LD50, rat, oral)
  • 5 mg · kg -1 ( LD50, mouse, ip)

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N- ethylmaleimide (NEM) is a derivative of the maleic acid imide. It is used in biochemical experiments, blocking of sulfhydryl groups, with which it reacts spontaneously, at pH values ​​of 6.5 to 7.5. This reaction can be monitored by measuring the decrease in light absorption at a wavelength of 300 nm. This allows the reagents or proteins which would otherwise interfere with the planned experiments are chemically inactivated.

CAS registry number PubChem DrugBank Dangerous Substances Directive (67/548/EEC) Intraperitoneal injection Biochemistry
589781
de