ABTS

• Diammonium -2, 2'- azino -di- (3- ethylbenzthiazoline -6 -sulfonic acid)
• 2,2 '- azino-bis - (3- ethylbenzthiazoline -6 -sulfonic acid)

Fixed

> 350

Poorly in water (20 g · l-1 at 20 ° C)

Attention

> 1,000 mg · kg -1 ( LD50, rat, oral)

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ABTS is the abbreviation of the diammonium salt of 2,2 '- azino- di ( 3- ethylbenzthiazoline -6 -sulfonic acid), a specific redox indicator, which is used primarily for glucose determination according to the glucose oxidase / peroxidase method. The free acid, a compound from the class of 2,2 '- azines can be easily split homolytically in stable radicals.

Use

ABTS is often used for biochemical studies because the radical formed from ABTS is photometrically easily detectable. Therefore, it can be used for example as a substrate for enzymes which are involved in the metabolism of free radicals. In this way, the kinetics can be studied. However, the antioxidant activity of a sample by means of this radical is quantified in so-called TEAC assay.

Due to its low toxicity, high temperature stability and the high extinction coefficient of ABTS is particularly suitable for high -throughput screening systems in microtiter plate format.

Proof

In oxidative aqueous environment ABTS forms a stable, green-colored radical cation, which can be measured photometrically at 405 nm.