Bicinchoninic acid

• 2,2 '- biquinoline -4, 4'-dicarboxylic acid
• 4,4 ' -dicarboxy- 2, 2'- biquinoline

Beige solid

Fixed

Template: Infobox chemical / molecular formula search available

Bicinchoninic acid (English bicinchoninic acid, BCA) is the common name for the chemical compound 2,2 '- biquinoline -4, 4'-dicarboxylic acid. Is a heterocyclic compound consisting of two linked in the 2- position Chinolinmolekülen each carrying carboxyl groups in the 4- position. Thus it is a dicarboxylic acid.

Properties

BCA reacts as biquinoline with monovalent copper ions to a complex compound with a violet color.

Use

BCA reaction in biochemistry to quantitatively, the photometric determination of protein (0.5 ug / ml - 1.5 mg / ml ) was used. This divalent copper ions react quantitatively with protein to monovalent copper ions. These come with the bicinchoninic acid a violet dye whose absorption can be measured photometrically at a wavelength of 562 nm.