Cleavable detergent

A cleavable surfactant is a surfactant which can be hydrolyzed under relatively mild conditions. Cleavable surfactants are used in the protein purification in preparation for mass spectrometry.

Properties

Residues of surfactants such as sodium lauryl sulfate ( SDS) or octyl glucoside, make the analysis of mass spectrometric data. The removal of the surfactants of the peptides to be tested, for example, by an in- gel digestion is often incomplete. In the electrospray ionization surfactants discomfort to the MALDI-TOF. In contrast, membrane proteins and lipids are water soluble only in the presence of surfactants. Therefore cleavable surfactants have been developed that hydrolyze under weakly acidic conditions, while the peptides remain unaffected, such as 3 - [3 - (1,1- Bisalkyloxyethyl ) pyridin- 1-yl ] propane -1-sulfonate (PPS ) PPS with methanol, 3 - ( (1 - ( furan-2- yl)- undecyloxy ) carbonylamino ) propane -1-sulfonate (protease MAX), 3 - [(2 -methyl-2 -undecyl -1 ,3-dioxolane 4-yl ) methoxy ]-1- propanesulfonate ( RapiGest SF).

Alternatives

Cleavable amphiphilic derivatives of α -cyano-4 -hydroxycinnamic acid ( CHCA ), which were coupled to formaldehyde of decanol, need not be removed since they can be used simultaneously as the matrix in MALDI -TOF -MS. Similarly, a matrix of α -cyano-4 -hydroxycinnamic acid with cetyltrimethylammonium bromide used in the MALDI-TOF -MS. There are matrix - mixtures that are less sensitive to surfactants.