Difference Gel Electrophoresis

The Difference Gel Electrophoresis (English for, difference gel electrophoresis ') is a method of quantity comparison of two or more samples of proteins in a 2D gel electrophoresis.

Properties

By a 2-D gel electrophoresis, the proteome can be represented, but a comparison of two samples in two different gels due to the inhomogeneities in the preparation of polyacrylamide gels, in the silver staining and the gel pieces in the extraction problem. Through a different marking both samples they can be simultaneously applied to a gel. The molecular marker is done by coupling different fluorophores at lysines or cysteines. Since Lysine most frequently occur in proteins, a marker satisfies less lysines per protein (german minimal labeling, minimum mark ' ), whereas cysteines are fully marked (English saturation labeling, marking saturation '). After electrophoresis, the proteins in the gel to be photographed with a fluorometer in each selected color, and then punched out individual proteins for further analysis. An identification is made of either one of Edman degradation or mass spectrometry after in-gel digestion.