Förster resonance energy transfer#BRET

The bioluminescence resonance energy transfer (English Bioluminescence resonance energy transfer, short BRET ) is a physical process of radiative energy transfer, which is based on the Förster resonance energy transfer ( FRET). In this process Biolumineszenzenergie is transferred free of radiation on a fluorescent dye ( acceptor fluorophore ). Since the intensity of bioluminescence resonance energy transfer, inter alia, depends on the distance of the Biolumineszenzquelle and the acceptor fluorophore of the bioluminescence resonance energy transfer is often referred to as an alternative to the fluorescence resonance energy transfer as "optical nanometer scale " used especially in biochemistry and cell biology. As Biolumineszenzquelle finds particular the enzyme Rluc, a luciferase from Renilla reniformis Seefedernart (common sea pansy ) using as its substrate coelenterazine h and acceptor fluorophore, the yellow fluorescent protein YFP application. A development of this conventional method is the BRET BRET2 method, which is based on the use of the substrate coelenterazine 400a ( Deep Blue C) and of the acceptor GFP2 ..