Guttavirus

The monotypic virus family Guttaviridae and its sole genus Guttavirus were newly created as a new Bakteriophagenspezies extrachromosomal in the investigation of genetic material in different strains of Sulfolobus Archaeengattung ( Crenarchaeota ) was isolated and characterized. In one strain ( Sulfolobus STH3 / 1, Sulfolobus neozealandicus ) this extremely thermophilic and acidophilic archaea, who came from a fumarole field south of Lake Taupo (New Zealand ), viruses were mixed with a drop -like shape (Latin gutta: drops ) was observed. The new virus species was described as Sulfolobus neozealandicus drop -shaped virus ( SNDV ).

The virions of SNDV are enveloped, irregular, predominantly oval or drop-shaped shaped capsids that have at its pointed end of a plurality of thin filaments. The function of these filaments is unknown. The symmetry of the capsid is also unclear appear in the electron microscope stacked helical structures. The virions are 110-185 nm long and a maximum of 70 to 95 nm wide. A major structural protein ( 17.5 kDa) and two minor structural proteins ( 13.5 and 13 kDa) are detectable in the virion.

The genome of the SNDV consists of a circularly closed, double-stranded DNA ( cccDNA ) and is about 20 kbp long. The genome is also methylated at very many places. Due to the special, not occurring in the host of N (6), methylation, it is assumed that the SNDV has its own DNA methyltransferase. The viral DNA is therefore compatible with most restriction endonucleases not cleavable only the dam methylation -dependent restriction enzyme DpnI to be cut in a position SNDV DNA.

Swell

  • W. Zillig et al.: Viruses, plasmids and other genetic elements of thermophilic and hyperthermophilic Archaea. FEMS Microbiol Rev (1996 ) 18 ( 2-3): pp. 225-236 (Review) PMID 8639330
  • HP Arnold et al. SNDV, a novel virus of the extremely thermophilic archaeon Sulfolobus and acidophilic. Virology (2000) 272 (2): pp. 409-416 PMID 10,873,785 ( full text )
  • G. Lipps: plasmid and viruses of the thermoacidophilic Sulfolobus crenarchaeote. Extremophiles (2006 ) 10 ( 1): pp. 17-28 PMID 16397749
  • Virus family
  • Bacteriophage
Virus classification National Center for Biotechnology Information Virus#Etymology DNA methylation DNA-Methyltransferase
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