Nitro blue tetrazolium chloride

3,3 ' - (3,3 '- dimethoxy-4, 4'- biphenylylene ) - bis [ 2 - (4 -nitro -phenyl) -5-phenyl -2H- tetrazolium chloride ]

Yellow crystals

Fixed

189 ° C.

  • Poorly in water

2000 mg · kg -1 ( LD50, mouse, oral)

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Nitro blue tetrazolium chloride (NBT ), a quaternary ammonium compound is a di - tetrazolium salt and redox dye.

Properties

By reducing the di- tetrazolium salt is first formed a red mono- formazan dye. Upon further reduction, a blue di- formazan dye formed.

Use

In biochemistry NBT in combination with 5 -bromo -4-chloro -3- indoxyl phosphate ( BCIP ) for sensitive detection of the enzyme alkaline phosphatase (AP ) is used. In medicine used NBT for diagnosis of chronic granulomatous disease.

CAS registry number PubChem Digital Object Identifier Analytical Biochemistry
606097
de