Nitrocefin

• (7R ) -3 - ( (E ) -2,4- Dinitrostyryl ) -7 - (2- thienylacetamido )-3 -cephem- 4-carboxylic acid

Yellow- orange solid

Fixed

167-169 ° C

Attention

Template: Infobox chemical / molecular formula search available

Nitrocefin is a substance selected from the chemical group of cephalosporins, which changes its color upon cleavage by β -lactamases from yellow to red.

Properties and Uses

The pure substance is readily soluble in dimethyl sulfoxide. It is sensitive to light. Your primary isolation was carried out from the discovered by Giuseppe Brotzu mold Acremonium.

Nitrocefin is a chromogenic cephalosporin: when the β -lactam ring is opened hydrolytically, the absorption maximum shifts from 390 nm to 486 nm, which goes to red with a color change from yellow accompanied. Nitrocefin is therefore suitable for the detection of bacteria that form β -lactamases. β -lactamases play an important role in the dreaded antibiotic resistance of bacteria. The test is more sensitive than the previously recognized on the basis of bromocresol test.