Pyranine
- 8 -hydroxy-1 ,3,6- pyrentrisulfonsäuretrinatriumsalz
- Trisodium 8- hydroxypyrene -1 ,3,6- trisulfonate
- Solvent Green 7
- C. I. 59040
- HOPSA
- HPTS
Yellow powder
Fixed
2.15 g · cm -3
> 300 ° C ( decomposition)
Slightly soluble in water ( 178 g · l -1)
Attention
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Pyranine is a fluorescent dye from the family of Pyrenfarbstoffe.
Properties
Pyranine is a yellow solid which is readily soluble in water. The solution shows an intense yellow-green fluorescence, whose intensity is dependent on the pH value.
Use
Pyranine is used as a fluorescent dye used to color hair, among other things, in soaps and highlighters. Moreover, it is to stain cleaning agents and as a marker liquid water circuits are used. For labeling experiments of waters, it is also permitted. The limit of detection in a commercial fluorescence spectrometer is approximately 5.10 -8 g · l-1, the emission peak at 512 nm
The dependence of the fluorescence of the pH value can be used in microbiology for the determination of intracellular pH.
Further Reading
- A. Fall, HJ Wygoda: Pyranin - a fluorescent dye for application- engineering experiments. (PDF, 620 kB) In: Nachrichtenbl German plants Schutzd. Volume 58, Number 3, 2006, pp. 79-85. ISSN 0027-7479
- JV Thomas, MR Brimijoin include: The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. In: Experimental eye research. Volume 50, Number 3, March 1990, pp. 241-249, ISSN 0014-4835. PMID 2,156,724th
- OF Mohammed, J. Dreyer, among other things: Solvent -dependent photoacidity state of pyranine Monitored by transient mid - infrared spectroscopy. In: ChemPhysChem: a European journal of chemical physics and physical chemistry. Volume 6, Number 4, April 2005, pp. 625-636, ISSN 1439-4235. doi: 10.1002/cphc.200400510. PMID 15,881,578th