Simulated body fluid

A simulated body fluid (English: Simulated body fluid, Abbr: SER) is similar to a solution with an ionic concentration of human blood plasma, where the same conditions prevail, such as the same pH and the same physiological temperature. SBF was initially by Kokubo et al. developed to analyze the changes on a surface of a bioactive glass ceramic.

Applications

Surface modification of metallic implants

Of synthetic material capable of binding to living bone is the formation of an apatite layer on the surface of the implant is of great importance. The SBF can be used as an in vitro test methods in order to explore the formation of the apatite layer on the surface of implants and thus predict in vivo Knochenbioaktivität. The consumption of calcium and phosphate ions in the SBF solution, resulting in spontaneous growth of bone-like apatite nuclei on the surface of biomaterials in vitro. Therefore, the apatite formation on the surface of biomaterials is true, soaked in the SBF solution as a successful development of new bioactive materials. The SBF technique for surface modification of metallic implants is usually a complex process, because there the preservation of uniform apatite layers on substrates takes at least seven days with daily refreshing the SBF solution to complete. Another method for the reduction of the coating time, the calcium and phosphate ions to concentrate in the SBF solution. Increased concentrations of calcium and phosphate ions in the SBF solution accelerates the coating process and eliminated in the meantime the need for regular replenishment of SBF solution.

Gene Therapy

An attempt was made to investigate the application of the SBF in gene therapy was. The calcium phosphate nano-particles, it is necessary for the delivery of plasmid DNA ( pDNA ), in the nucleus of the cells, were synthesized in a SBF solution and mixed with pDNA. It showed higher in vitro studies Genlieferungs efficiency for the calcium-phosphate/DNA complexes produced by the SBF solution than that produced in pure water ( control ).