Thiobarbituric acid
- 2- thiobarbituric acid
- 2 Thioxodihydropyrimidin -4, 6 (1H, 5H) -dione
235 ° C.
Readily soluble in hot water (50 g per liter at 95 ° C)
Attention
> 5000 mg · kg -1 ( LD50, rat, oral)
Template: Infobox chemical / molecular formula search available
Thiobarbituric acid is a derivative of the barbituric acid. It is used as a reagent in analytical chemistry.
Properties
Thiobarbituric acid forms white crystals with an unpleasant odor. In hot water, the compound is readily soluble.
Use
In analytical chemistry thiobarbituric acid used as a reagent to sorbic acid, and the determination of the biomarker malondialdehyde ( MDA). The TBA assay ( TBA = thiobarbituric acid) based on the reaction of thiobarbituric acid with oxidation products of lipids, such as malondialdehyde. In this reaction, a pink dye is formed from one molecule of the bifunctional aldehyde with two molecules of thiobarbituric acid, which can for example be determined by photometry quantitatively.
Further Reading
- Lykkesfeldt J.: Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet -visible spectrophotometry. In: Clinical Chemistry Volume 47, Number 9, September 2001, pp. 1725-1727, ISSN 0009-9147. PMID 11,514,418th
- R. Guillén - Sans, M. Guzmán - Chozas: The thiobarbituric acid (TBA ) reaction in foods: a review. In: Critical reviews in food science and nutrition Volume 38, Number 4, May 1998 pp. 315-330, ISSN 1040-8398. PMID 9,626,489th
- Janero DR: Malondialdehyde and thiobarbituric acid- reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. In: Free radical biology & medicine Volume 9, Number 6, 1990, pp. 515-540, ISSN 0891-5849. PMID 2079232nd
- A. Kolakowska, J. Deutry: Some comments on the usefulness of 2 - thiobarbituric acid (TBA ) test for the evaluation of rancidity in frozen fish. Volume 27, Number 5, 1983, pp. 513-518. doi: 10.1002/food.19830270530